Coding
Intein
Part:BBa_K1483003:Design
Designed by: Nikolas Layer, Philipp H. O. Mayer and Philip Roessler Group: iGEM14_Tuebingen (2014-10-06)
Ssp GyrB Split Intein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Part was designed in RFC25, since it is specifically used for fusion of peptides
Source
Intein of DNA grase subunit B of the cyanobacterium Synechocystis species, strain PCC6804. Sequence optimized for codon usage in E. coli K12 and synthesised.