Project

Part:BBa_J54252:Design

Designed by: Yusaku Nakashima   Group: iGEM06_Tokyo_Alliance   (2006-10-21)
Revision as of 05:23, 23 October 2006 by Yusaku (Talk | contribs) (→‎Design Notes)


LacITandem_regulated(B-5-2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 812


Design Notes

We inserted two lacI_Binding_site tandemly because just one site did not work well.

Source

synthesis

References