Generator

Part:BBa_K1316015

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-29)
Revision as of 07:01, 29 September 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pRhamnose regulating csgB and csgA curli proteins

This part is meant to express the csgB and csgA curli genes, which respectively code for the production of the curli protein that is anchored to the cell membrane (CsgB); and for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure.

The expression of these two genes will, then, occur at the same time and under the same conditions (in the presence of Rhamnose in this case)

This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None