Generator

Part:BBa_K1486002

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-12)
Revision as of 17:46, 13 October 2014 by Ionepla (Talk | contribs)

No part name specified with partinfo tag.

Purpose of the Unit

This unit (in the control layer) is a button used to apply pressure on the cells in the chamber. It was designed to decrease the outflow of cells into the channels.

Associated Biobricks

In the context of the same experiment, we designed another construct with the sfGFP moiety attached to the C terminus of CpxR: https://parts.igem.org/Part:BBa_K1486005.



Functional Parameters

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Categories
//cds/reporter/gfp
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//direction/forward
//function/regulation/transcriptional
//function/reporter/fluorescence
//promoter
//rbs/prokaryote/constitutive
//regulation/multiple
regulator
transcriptional
Parameters
biology
chassisEscherichia coli
colorGreen
controlAraC, arabinose
directionForward
emission510
excitation485
functionTransciption regulator
negative_regulatorsCpxA (phosphatase activity)
positive_regulatorsCpxA (Histidine kinase activity when activated)
rbsElowitz
resistanceChloramphenicol