Coding

Part:BBa_K1420005

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-10-09)
Revision as of 02:09, 10 October 2014 by Tarnowki.jm (Talk | contribs)

merT, mercuric transport protein

merT encodes a transmembrane mercuric binding protein

BBa_K1420005 long Not understood

merT is found in the Serratia marcescens plasmid pDU1358 on the other side of the bidirectional promoter opposite of merR. It is 351 base pairs long and it codes for a mercuric transport protein.


Structure

Structure of merT can not be obtained due to the difficulty in obtaining the crystalline structure of a transmembrane protein.


Mechanism The transport mechanism of ionic mercury from the bacterial cell periplasm to cytosplasm is not completely understood. However, it is thought that in cooperation with merP, a periplasmic mercury binding protein, Hg 2+ is transferred from merP to the first of the three transmembrane helices onto two cysteines within merT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 45
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 30


[edit]
Categories
//binding/metal
//cds
Parameters
None