Part:BBa_K1355004

Mercury ions bioremediating device

The biorremediator of mercury device is composed by our “Essential Biobrick” (BBa_K1355001) - a bidiretional promotor regulated by the MerR protein + a MerA translational unit (BBa_K1355000) - a strong RBS + mercury ion reductase + double terminator BBa_B0015, represented below:

In presence of mercury, MerR protein will bind to it so MerT, MerP and MerA protein will be expressed and bioremediation will start! MerP and MerT proteins are responsible for transporting mercury from the periplasm to cytoplasm, leading it to mercury ion reductase (MerA)! The beautiful MerA is responsible for reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave the bacteria.

Figure 1: Mercury Bacter Hg bioremediator (DH5-alpha transformed with BBa_K1355004)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 988
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 586
  Illegal NgoMIV site found at 1160
  Illegal NgoMIV site found at 2397
  Illegal NgoMIV site found at 2459
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 579