Tag
USP45

Part:BBa_K1381018

Designed by: Alexander Wirtanen, Niklas Handin, Marcus Hong and Jonas Mattisson   Group: iGEM14_Uppsala   (2014-10-03)
Revision as of 14:53, 12 October 2014 by Amaron (Talk | contribs)

B0034-USP45-(GS)x5

USP45 USP45 is en export tag that works in lactobacillus as well as E.coli.

The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part contains the RBS and export tag USP45. In team Uppsala iGEM 2014 project this part was to be copled with the colicin FyBBa_K1381017. It contains an AgeI restriction site for a more easily fusion with other protein sequence.(The Colicin Fy BBa_K1381017 part was designed with a MgoMIV site) It does not follow the Freiburg Biobrick standard 25 since it does not have an MgoMIV site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None