Part:BBa_K1341001

PATH WAY TEST SYS IN BIO-COMPASS

This system has proved that we have successful insert the BBa_K1341000 in the Afl III enzyme site(which has designed in the No.5 node sequence before this path way linked).Besides, this system also have a BamH I site in the No.2(start) point, a Nco I site in the No.8(terminal） point. The TetR device (BBa_K1341003) and LacI device (BBa_K1341002) can insert in this measurement system according to the requirement. By using the aTc and IPTG, we can prove whether the path way is the right result exactly such as this(2-4-5-7-8) CHA TU PIAN 1

characterization

The analysis about the logic of the system CHA TU PIAN 2

We can prove that the GFP signal part(BBa_K1341000) of the screen device has been insert in our path way test system((BBa_K1341000 is almost 1000bp, and the path way sequence is about 200 bp, this Electrophoresis result is about 1200bp), and we make this gene circuit(BBa_K1341001) do the follwing test about whether this path way circuit is the right way we want.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 11
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 180
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 837