Regulatory

Part:BBa_K091101

Designed by: Erin Feeney   Group: iGEM08_Davidson-Missouri_Western   (2008-05-29)
Revision as of 13:56, 2 June 2008 by ErFeeney (Talk | contribs)

pTet_Lac hybrid promoter

This promoter is expected to be repressed by tetR and lacI. It contains two binding sites for TetR and one LacI binding site. The part is a predicted AND logic gate; transcription requires the presence of both aTc AND IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//regulation/multiple
Parameters
control
directionForward
negative_regulators2
o_h
o_l
positive_regulators