Regulatory
yenbox_J23

Part:BBa_K1381001

Designed by: Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg   Group: iGEM14_Uppsala   (2014-10-02)
Revision as of 13:16, 2 October 2014 by Registry (Talk | contribs)

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yenbox_J23113

yenbox_J23113 consists of the Anderson promoter J23113 fused togheter upstreams of a luxbox homoloug, called the yenbox. When interaction between the yenbox and the activator YenR occur, the base level of expression of the promoter should be induced. But this has been shown not to work. Probably the changed bases in the end of the yenbox, prevent the interaction between YenR and the yenbox from happening.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None