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Device

Part:BBa_K1355002

Designed by: Maria Clara Astolfi, Luna Barroco de Lacerda   Group: iGEM14_UFAM_Brazil   (2014-10-06)
Revision as of 21:32, 11 October 2014 by Mctastolfi (Talk | contribs)

Mercury ions detector device


We used the biobrick (BBa_E0840) available on iGEM database since 2004 to connect to our “essential bio brick” (BBa_K1355001) and - together - form a system capable of bio detection mercury in bacteria, represented below:

L4.jpg

In the presence of mercury, MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and bio detection starts! MerP and MerT proteins are responsible for the transport of mercury from the periplasm to cytoplasm and GFP will act as a reporter alerting the presence of mercury in the middle and higher is mercury’s concentration, higher will be GFP expression. And if there isn’t mercury on medium, MerT, MerP and GFP synthesis will be repressed by MerR repressor protein.

Biosensor.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 1160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1862
    Illegal SapI site found at 579


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