Regulatory
FZ

Part:BBa_K1442006

Designed by: Caroline de Cock   Group: iGEM14_Warwick   (2014-08-14)
Revision as of 19:48, 11 October 2014 by CarriedC (Talk | contribs)

Anti-theophylline Aptazyme

An aptazyme is an allosteric ribozyme acting as a regulatory riboswitch. It self-splices in response to theophylline, a drug used to treat asthma and other chronic obstructive pulmonary diseases acting as a bronchodilator. This differs from caffeine by one methyl group and a nitrogen atom creating the structure seen below. This is not endogenous to the body therefore we used this element as a "kill switch" for our system.

One RNA molecule binds to theophylline with a dissociation constant of 0.1microM and Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
Parameters
None