Translational_Unit

Part:BBa_K1497003

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-11)
Revision as of 10:17, 11 October 2014 by SaHein (Talk | contribs)

B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

The chalcone isomerse catalyzes the reaction from naringenin chalcone to naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.

The iGEM Team TU Darmstadt 2014 used the CHI and verified the function of the CHI in their naringenin operon (K1497007).


Figure 1 Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. The CHI has a high E-value and produce only S-naringenin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
n/aB0034-CHI - Chalcone Isomerase from Petunia with strong RBS