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Coding
Part:BBa_K1497010
Designed by: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky Group: iGEM14_TU_Darmstadt (2014-10-07)
Dihydroflavonol 4-reductase (DFR)
PDZ-Domain
The dihydroflavonol 4-reductase (DFR; EC 1.1.1.219) from the plant Dianthus caryophyllus is an enzyme, catalyzing the reversible conversion of dihydroflavonols e.g. dihydro-kaempferol or dihydroquercetin into their corresponding leucoanthocyanidin. This reaction is NADPH-dependent (Liew et al. 1998) but its counter reaction can also occur with NAD+ instead of NADP+ (Queen Mary University of London 2014). DFR contains 353 amino acids and has a molecular weight of approximately 39.4 kDa. |
Figure 1 Reaction of the DFR. One |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 67
References
Chye-Fong Liew, Chiang-Shiomg Loh, Chong-Jin Goh, Saw-Hoon Lim 1998, 'The isolation, molecular characterization and expression of dihydroflavonol 4-reductase cDNA in the orchid, Bromheadia finlaysoniana', Plant Science, vol. 135, no. 2, pp. 161-169. Available from: ScienceDirect (31.08.2014)
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Categories
Parameters
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
//cds/biosynthesis/anthocyanins
n/a | Dihydroflavonol 4-reductase (DFR) |