Generator

Part:BBa_K1355001

Designed by: Maria Clara Tavares Astolfi, Luna Barroco de Lacerda   Group: iGEM14_UFAM_Brazil   (2014-10-06)
Revision as of 20:41, 10 October 2014 by Lunalacerda (Talk | contribs)

Regulation and transport of mercury ions

We call this part an “Essential biobrick”. We designed it meaning to be the key piece of many genetic construction related to mercury, eg. Bio-sensor, bio-remediator, bio-accumulator, and among others. This biobrick has bidirectional promoter, having dual functions: a. in a reverse MerR regulator conjugated with a terminator from the tryptophan operon, and B. a forward MerP and MerT transporters, as represented below:

L3.jpg


Source

This construction is based on sequences present in the O26-CRL plasmid found in Escherichia coli O26. We added a reverse tryptophan operon terminator to ensure transcription termination of the merR messenger RNA. However we didn’t add transcription terminator after the merP gene, aiming connect this biobrick with others, enabling various functions related to mercury (eg. Bio-sensor, bio-remediator, bio-accumulator)

[edit]
Categories
//binding/metal
Parameters
None