Part:BBa_K1455006

psid - pTet - AmilCP - pag activator (PSP3) Positive feedback

This device is a positive feedback for itself, producing more amilCP (blue chromoprotein) each time.

AmcilCP and PSP3 are expressing constitutively by pTet promoter, which can be turned off by the prescence of TetR wtih no tetracycline. PSP3 is an activator for psid promoter, which is upstream pTet, thus activates AmilCP and PSP3 again and again.

In addition, PSP3 does not only activate genes downstream of psid, it also serves as activator for other five promoter of the same family (parts BBa_I746360 to BBa_I746365).

Is important to highlight that TetR is, theoretically, able to turn off all the system, and after the system can be turned on again by the addition of tetracycline (or analogues), so this device could be useful as a part of a more complex regulation circuit.

Here is an example of an E: coli DH5α culture carrying this device:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]