Part:BBa_K1497019

fdeR (Naringenin binding protein) and fde operon regulatory domain FdeR is a homo dimeric protein from Herbaspirillum seropedicae. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.

We modified the naringenin biosensor from Siedler et al. by elimination of the internal EcoRI restriction site and replaced the CFP by the Biobrick Suffix. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.

Homology model of FdeR. PDB: 2ESN was used as template.

=Usage and Biology

You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.

You can use the reporters for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:

GFP K1497020

mKate K1497021

CFP K1497022

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 95
  Illegal NgoMIV site found at 452
  Illegal NgoMIV site found at 543
  Illegal NgoMIV site found at 555
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 271