Composite
p-luxODGFP

Part:BBa_K1455002

Designed by: Camilo Gómez Garzón   Group: iGEM14_Colombia   (2014-10-07)
Revision as of 16:35, 7 October 2014 by Registry (Talk | contribs)

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pBAD - luxO D47E - GFP

Postivie control by fluroescence of LuxOD47E under arabinose-inducible promoter.

LuxOD47E acts like phosphorilated LuxO (Vibrio cholerae quorum sensing system).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 292
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 980
    Illegal BsaI.rc site found at 2189
    Illegal SapI.rc site found at 942


[edit]
Categories
Parameters
None