Protein_Domain
Gp41-1(N)

Part:BBa_K1362412:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-06)
Revision as of 20:56, 6 October 2014 by Jakob (Talk | contribs) (→‎Source)


Gp41-1 N-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.


Source

Part information and data come from Schmidt et al. [1]. The sequence was acquired from Pietrokovski et al. [1] Part DNA was obtained by DNA synthesis.

References

[1] 1. Carvajal-Vallejos, P., Pallissé, R., Mootz, H. D. & Schmidt, S. R. Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J. Biol. Chem. 287, 28686–96 (2012).

[2] Bareket Dassa, Nir London, Barry L. Stoddard, Ora Schueler-Furman, Shmuel Pietrokovski Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family Nucleic Acids Res. 2009 May; 37(8): 2560–2573. Published online 2009 March 5. doi: 10.1093/nar/gkp095