Measurement

Part:BBa_K1403000:Design

Designed by: Urszula Czerwinska   Group: iGEM14_Paris_Bettencourt   (2014-10-03)
Revision as of 16:25, 6 October 2014 by Ula (Talk | contribs) (→‎Design Notes)


GFP generator in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

The designed was made according to interlab study requirements. The promoter and the vector have been taken from BBa_K823005, cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from BBa_E0240 also with XbeI and SpeI and introduced to the cut promoter + backbone. We used heatshock competent NEB cels. For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]

Source

kit 2014 Plate 1, Well 20K Plate 2, Well 24B

References