Device

Part:BBa_K1350006:Experience

Designed by: Yiting Luo   Group: iGEM14_SZU-China   (2014-09-28)
Revision as of 11:59, 6 October 2014 by Yitingluo (Talk | contribs) (Characterization experiment on BBa_K1350006--Mdfa)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1350006

User Reviews

UNIQ8def6ebeb8cd82cf-partinfo-00000000-QINU UNIQ8def6ebeb8cd82cf-partinfo-00000001-QINU


== Characterization experiment on BBa_K1350006--Mdfa ==

 Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10.
 We performed some experiments to prove that the Mdfa really founctions.
 We performed several experiments with different LB medium. We used: 
 
 3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5
 3o without mdfa in LB  pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5