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Part:BBa_K1467100:Design

Designed by: Cara Deal, Jessica Gray   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)
Revision as of 10:12, 24 September 2014 by Registry (Talk | contribs)

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RFP coding device - Golden Gate Module Flipper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1082
    Illegal BsaI.rc site found at 6


Design Notes

The sequences flanking the RFP part contain the recognition sequence for BsaI and the (separate) cut-site for BsaI. The cut sites produce 4base pair 5' overhangs compatible with GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules.


Source

Part:BBa_J04450

References