Coding

Part:BBa_K1351009:Design

Designed by: Mona Dotzler   Group: iGEM14_LMU-Munich   (2014-10-05)
Revision as of 14:59, 5 October 2014 by Registry (Talk | contribs)

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57 aa flexible protein linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 95


Design Notes

The linker sequence corresponds to the linker used in Liew et. al., 2012.

Oligonucleotides were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3


Source

This part was generated by annealing synthesized oligonucleotides followed by ligation in pBS1C3:

L56_ENX_SD_Ngo_precutE_fwd1: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG ATCGATCCGGCAGGAAGTAGTCCGTCGACA

L56_fwd2: GAGCTTGCATTTGACGAAGAAGACGACGATGATGAAGAGCCTGAAGATGCAGGCCCGGGAGATGGTGTGCTCGGATTCGAA

L56_SNP_Age_precutP_fwd3: TTCGTCAAATGCAAGCTCCGGCACGACGACTACACCTGATCTTCCTGGGTCACCG ACCGGTtaatACTAGTagcggccgCTGCA

L56_SNP_Age_precutP_rev1: GcggccgctACTAGTattaACCGGT CGGTGACCCAGGAAGATCAGGTGTAGTCGTCGTGCCG

L56_rev2: GAGCTTGCATTTGACGAAGAAGACGACGATGATGAAGAGCCTGAAGATGCAGGCCCGGGAGATGGTGTGCTCGGATTCGAA

L56_ENX_SD_Ngo_precutE_rev3: GGAGTACTAGGTCCCTCAGGTGTCGACGGACTACTTCCTGCCGGATCGAT CATGCCGGCttcctccttacTCTAGAagcggccgcG


References