Coding

Part:BBa_K1351008:Design

Designed by: Mona Dotzler   Group: iGEM14_LMU-Munich   (2014-10-05)
Revision as of 12:55, 5 October 2014 by Registry (Talk | contribs)

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Signal peptide of B. subtilis endonuclease YhcR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native signal peptide of YhcR was used instead of known Sec-dependent signal peptides as Liew et. al. did 2012.


Source

This part was generated by amplification from B. subtilis W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

SP_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGCTGTCTGTCGAAATGATAAGC

SP_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT AGCTTCGAACGTGTACATTACATTTAAG

References