RBS
T7 RBS

Part:BBa_K1362090

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-03)
Revision as of 02:10, 5 October 2014 by Jan glx (Talk | contribs) (fixed citation)

RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.

The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli.


1. Olins, P. O. & Rangwala, S. H. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. J. Biol. Chem. 264, 16973–6 (1989).

[edit]
Categories
//chassis/prokaryote/ecoli
//rbs/prokaryote
//rbs/prokaryote/constitutive
//regulation/constitutive
//ribosome/prokaryote/ecoli
Parameters
None