Part:BBa_J61002

EX-Ptet-S-rbsRFP-P "RFP reporter"

Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.

ApE file:{pBca1020-R0040}

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal NotI site found at 878
  Illegal NotI site found at 2933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal AgeI site found at 581
  Illegal AgeI site found at 693
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1966