Regulatory
Part:BBa_K1402010
Designed by: Chen Xi Group: iGEM14_NJU-QIBEBT (2014-09-29)
Lac Promoter
This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: repressed by LacI induced by lac or IPTG, showing a high expression level. CAP protein will not influence the expression. Strong promoter. Only E.Coli BL21(DE3) can use this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//collections/biofab/promoter
//direction/forward
//dna/nucleotide
//direction/forward
//dna/nucleotide
biology | |
control |