Part:BBa_K1470001
puromycin N-acetyl-transferase
Puromycin is an antibodic derived from the bacteria Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import [1]. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.
A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. The nascent chain containing puromycin leads to a translational stop.
Streptomyces alboniger protects itself from puromycin with an acetyl transferase. It uses acetyl-CoA to bind the acetyl moiety to the amino group. In this state puromycin cannot be recognized by the ribosom anymore [2].
This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99 % of uninfected cells will be dead within two days.
References
[1] NEUBERT, D. AND LEHNINGER, A. L.: The effect of oligomycin, gramicidin, and other antibiotics on reversal of
mitochondrial swelling by adenosine triphosphate. Biochim. biophys. Acta 62: 556-565, 1962.
[2] DARKEN MA.: PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS. Pharmacol Rev. 1964 Sep;16:223-43.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 481
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