![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1365006:Design
Designed by: Sandra Mous Group: iGEM14_Groningen (2014-09-24)
NisR and NisK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3' to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix.
Changed the GTG start codon of NisR into a ATG.
Source
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.