Coding
MSA
Part:BBa_K1392932:Design
Designed by: Burak Kızıl Group: iGEM14_METU_Turkey (2014-09-29)
4-hydroxy-2-ketovalerate aldolase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 813
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to take 4-hydroxy-2-ketovalerate aldolase from Escherichia coli BL21(DE3)'s complete genome a primer has designed and to get 1014 bp, the part has connected to CHL backbone.
Source
Escherichia coli BL21 (DE3)