Coding

Part:BBa_J52223

Designed by: Monika Ciglic   Group: iGEM06_Slovenia   (2006-09-16)
Revision as of 16:51, 16 September 2006 by Registry (Talk | contribs)

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rluc-linker-PEST191

rluc-linker-PEST191 is made of rluc (which act as a reporter) connected with PEST191 (2h-degradating sequence)through linker that codes for six amino acids

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1459
    Illegal PstI site found at 1473
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1459
    Illegal PstI site found at 1473
    Illegal NotI site found at 7
    Illegal NotI site found at 1466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1459
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1459
    Illegal PstI site found at 1473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 568
    Illegal SapI site found at 996


[edit]
Categories
Parameters
None