Part:BBa_K1445002

High copy BioBricked M13 phagemid vector

This biobrick backbone has the high copy number pUC19 origin of replication and an Ampicillin resistance marker.

Part also contains the M13 origin of replication which allows the phagemid to be taken up by the M13 phage. Vector does not contain any expressible phage genes; therefore, a Helper Phagemid is required for phage production.

Vector contains the following:

   iGEM VF2 and VR sequencing sites

   T7 promoters that flank the MCS

   Translational stop sequences (BBa_B0042) that flank the MCS

   Transcriptional terminator BBa_B0055 upstream of the MCS

   Transcriptional terminator BBa_B0054 downstream of the MCS

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2990
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2996
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2990
  Illegal BglII site found at 2852
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2990
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2990
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3005
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1240