Composite

Part:BBa_K1499500:Design

Designed by: Poorwa Godbole   Group: iGEM14_StanfordBrownSpelman   (2014-08-25)
Revision as of 01:39, 15 September 2014 by Pgodbole (Talk | contribs) (→‎Source)

quorum sensing machinery that activates GFP expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1815
    Illegal BsaI.rc site found at 2543


Design Notes

Since the luxI gene is under the expression of a lac-repressible promoter, in order to control expression of GFP, this construct must be used in lac deficient cells.


Source

Combination of two previous parts: BBa_I13202 [1] and BBa_T9002 [2].

References

[1] [2]