Coding
REACh1

Part:BBa_K1319001:Design

Designed by: Michael Osthege, Florian Gohr   Group: iGEM14_Aachen   (2014-09-03)
Revision as of 19:31, 3 September 2014 by Registry (Talk | contribs)

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RFC[25]-compatible dark quencher based on K1319000 (E0030)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Y145W mutation was introduced to eliminate fluorescence of the protein.


Source

The part was created by site-directed mutagenesis on K1319000 that we previously created from E0030

References