Regulatory

Part:BBa_K1346010

Designed by: Derrick Lee   Group: iGEM14_UCSF_UCB   (2014-08-12)
Revision as of 21:24, 3 September 2014 by Jchsueh (Talk | contribs)

pCLG1

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.

CLG1-zoomed.png

Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized, meaning pCLG1 is not very responsive to alpha factor.

140722 AFRP GFP DB ROUND 4 zoomed.png


AFRP GFP DB ROUND 4 140722.png

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None