Regulatory

Part:BBa_K1346000

Designed by: Eleanor Amidei   Group: iGEM14_UCSF_UCB   (2014-07-15)
Revision as of 20:39, 3 September 2014 by Jchsueh (Talk | contribs)

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

140630 pTEF1 GFP ROUND 1.png

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


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