![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1460003:Design
Designed by: Eric Holmes Group: iGEM14_Cornell (2014-08-14)
Anderson Promoter + NixA + Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Anderson promoter provides strong constitutive expression of NixA in E. coli.
Source
Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.