Generator

Part:BBa_K1486005

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-13)
Revision as of 12:30, 13 August 2014 by Registry (Talk | contribs)

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Arabinose promoter + sfGFP-CpxR [Cterm]

This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1961


[edit]
Categories
//cds/reporter/gfp
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//direction/forward
//function/regulation/transcriptional
//function/reporter/fluorescence
//promoter
//rbs/prokaryote/constitutive
//regulation/multiple
//terminator/double
regulator
transcriptional
Parameters
biology
chassisEscherichia coli
colorGreen
controlAraC, arabinose
directionForward
emission510
excitation485
functionTransciption regulator
negative_regulatorsCpxA (phosphatase activity)
positive_regulatorsCpxA (Histidine kinase activity when activated)
rbsElowitz
resistanceChloramphenicol