Regulatory

Part:BBa_K1346003

Designed by: Derrick Lee   Group: iGEM14_UCSF_UCB   (2014-08-05)
Revision as of 21:17, 5 August 2014 by Derrlee (Talk | contribs)

pTEF1(m10) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

140630 pTEF1 GFP ROUND 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


[edit]
Categories
Parameters
None