Regulatory

Part:BBa_K1346000

Designed by: Eleanor Amidei   Group: iGEM14_UCSF_UCB   (2014-07-15)
Revision as of 18:59, 15 July 2014 by Elamidei (Talk | contribs)

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than M7.

140630 pTEF1 GFP ROUND 1.png

data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


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