Device

Part:BBa_J119349

Designed by: Kelly Cochran   Group: Eckdahl Lab   (2014-06-30)
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Ammeline Biosensor with Riboswitch D2 + RFP

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (D2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The D2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAmmeline Biosensor with Riboswitch D2 + RFP