Composite

Part:BBa_K1300000

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-16)
Revision as of 01:20, 21 June 2014 by J.reyes.97 (Talk | contribs)

recA promoter + Key (taRNA)

recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.


Sequencing results compared with expected DNA sequence Recaplustrans.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 198


[edit]
Categories
//classic/regulatory/other
Parameters
None