Composite

Part:BBa_K1255003

Designed by: Antonio Cardenas, Irina Romero, Dulce Valdez   Group: iGEM14_CIDEB-UANL_Mexico   (2014-06-16)
Revision as of 13:30, 17 June 2014 by Marea (Talk | contribs)

pConst + Riboswitch + BSMT1

BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.

BSMT1 (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None