Part:BBa_K1073022:Experience

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Applications of BBa_K1073022

The iGEM Team Braunschweig 2013 used this device as a reporter to distinguish two different strains when cultivated in a mixed culture.
When eforRed is expressed, the colonies on agar plates as well as the liquid culture exhibit a bright pink color.

Part Sequence Quality

This part's real sequence may end with two stop codons, TAATAA. This is not listed in [the original source part's sequence https://parts.igem.org/Part:BBa_K592012] or in this part's sequence, but sequencing from the registry indicates it may be present. Beware.

User Reviews

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••••• iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoprotein during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm. iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm. Absorption maximum of eforRed: 534 nm iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence. Emission maximum of eforRed: 605 nm iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.

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