Part:BBa_K624051

pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP

pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP

pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.

Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

All of the ribosome binding sites are from Psmp3 with 6 bps truncated.(BBa_K624013).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2558
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3555
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 898
  Illegal NgoMIV site found at 4172
  
• 1000
  COMPATIBLE WITH RFC[1000]