Reporter

Part:BBa_J97000

Designed by: Drew Endy   Group: BioFAB   (2014-02-04)
Revision as of 16:00, 6 February 2014 by Kim (Talk | contribs)

IP-Free EiraCFP (Cyan Fluorescent Protein)

This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement.

  • Excitation 398nm, Emission 498nm
  • Molecular Weight: 26.0kDA
  • Length: 233 amino acids

EireCFP appears colorless to the naked eye, but has a light blue color under UV light.

EireCFP
Excitation and Emission Spectra

Usage and Biology

DNA 2.0 created a poster to show how the parts were created which is available on their site here.

DNA 2.0 IP-Free Fluorescent Protein Collection

Part BBa_J97000 is a member of a collection of four IP-Free fluorescent proteins. The four members of the collection have the following part numbers:

  • EiraCFP: BBa_J97000
  • JuniperGFP
  • BlazeYFP
  • TannenRFP

DNA2.0’s IP-Free© synthetic non-aequorea fluorescent proteins are intended to be used as a source of different fluorescent protein coding sequences (genes) that can be amplified by PCR and cloned into any other expression vector of choice.

Protein Collection Images under fluorescent light Protein Collection Excitation and Emission Information

This part was contributed under the BioBrick Public Agreement (BPA). Click on the BioBrick Foundation logo to see more information about the BPA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

emission498
excitation398
[edit]
Categories
//cds
//cds/reporter/cfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//legal/ip-free
Parameters
emission498
excitation398