![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
TALEA-dife
Part:BBa_K1189021
Designed by: Chris Wintersinger, Denny Hoang, Taylor Remondini Group: iGEM13_Calgary (2013-09-17)
Fusion of ferrtin subunits linked to DNA detectors
Ferritin is a protein shelled nanoparticle and is composed of a mixture of 24 light (BBa_K1189024) and heavy (BBa_K1189025) subunits. It is ubiquitous across eukaryotic and prokaryotic systems and is used to sequester intracellular iron (Chasteen et al., 1991). The 2013 iGEM Calgary used ferritin’s iron core as a reporter and its protein shell to scaffold DNA sensing TALEs as part of their project, the FerriTALE (see Figure 1).
![](https://static.igem.org/mediawiki/parts/0/0e/L-Ferritinh-FerritinHis-TALE_APlacI.png)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3656
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Categories
Parameters
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