Part:BBa_K1189025
Heavy chain human ferritin
This heavy ferritin chain comes from humans. This part along with light ferritin (BBa_K1189024 P02794 [UniParc]), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits (Lawson et al., 1991). The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures. The difference between light ferritin is that this chain contains a ferroxidase centre. Protein domains which orient toward the core of ferritin molecules cause the oxidation of intracellular iron from Fe^2+ to Fe^3+ to initiate formation of a ferrihydrite core.
Figure 1. Ribbon visualization of a fully assembled ferritin protein.
This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS (Zhang et al., 2013).
This part was primarily used to construct BBa_K1189019 in order to allow us to express and purify the protein.
Applications of BBa_K1189025
This part used primarily for the construction of parts containing the heavy ferritin subunit (BBa_K1189018 BBa_K1189019 BBa_K1189021).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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