Composite

Part:BBa_K1104102:Design

Designed by: Yin-Chih Chen   Group: iGEM13_NYMU-Taipei   (2013-10-29)
Revision as of 21:09, 29 October 2013 by Ericire (Talk | contribs)

pLac+RFP-FimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. During blunt-end ligation, we should add a kinase, in order to phosphoylate the two ends of the DNA that we cloned. The ligase can only work when the DNA is phosphorylated.
2. After ligation, it is a must to do a PCR check to see if you have ligated the coding sequence in the right direction.


Source

This part is ligated from different sources: 1. Escherichia coli K-12 substrain MG1655 genomic sequence
2. Biobrick sent from the headquarter

References

1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH]
2. Wellens, A., Lahmann, M., Touaibia, M., Vaucher, J., Oscarson, S., Roy, R., Remaut, H., ... Bouckaert, J. (January 01, 2012). The tyrosine gate as a potential entropic lever in the receptor-binding site of the bacterial adhesin FimH. Biochemistry, 51, 24, 4790-9.