Composite

Part:BBa_K1021017

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)
Revision as of 02:25, 29 October 2013 by A.Han (Talk | contribs)

PT7+crtB

This part is the crtB gene (the second component of the carotenoid biosynthesis pathway) downstream of the T7 promoter.


Caropath.png
Adapted from KEGG reference pathways

crtB was originally from the 2008 Edinburgh team that isolated the coding sequence of crtB from Pantoea ananatis. crtB encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).

The T7 promoter system was used in order to express multiple genes simultaneously. Fungal promoters are quite long, and prokaryotes have systems to splice out long repetitive sequences of DNA. Because the T7 is a short sequence, each of the carotenoid genes could be put behind the T7 promoter for maximum simultaneous transcription of the carotenoid genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None